Coding

Part:BBa_K3505005:Design

Designed by: Asteria Tsapadikou   Group: iGEM20_Thessaly   (2020-10-09)


TetR- Tet repressor GB compatible with B3-B5


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BpiI sites in order to be compatible with GoldenBraid and MoClo. In the following sequence there are two BtgZI sites. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B3-B5.


Source

E.Coli , sequence found through Addgene https://www.addgene.org/97094/

References